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. 2016 Sep 30;11(9):e0163920. doi: 10.1371/journal.pone.0163920

Table 1. Primers for the construction of recombinant expression plasmids.

Name Sequence (5′-3′) Enzyme
F-GST-N1 CAGGATCCGCCAACCAGGGACAACGT Bam HI
R-GST-N1 CACTCGAGGAATTTCAAAGCTTTGGAT Xho I
F-GST-N2 CAGGATCCGATGGTAAAGTGCCAGGC Bam HI
R-GST-N2 CACTCGAGAGTTCTCTTCCAGGTGTGTT Xho I
F-GST-N3 CAGGATCCGCAGGTAAAGGTGATGTGA Bam HI
R-GST-N3 CACTCGAGGTTCGTTACCTCATCAATCA Xho I
F-GFP-N GTGAATTCATGGCCAACCAGGGACAACGT Eco RI
R-GFP-N GTGGATCCTTAGTTCGTTACCTCATCAATCA Bam HI
F-TGEV-N-565-606 AATTCAGTGTAGAACAAGCTGTTCTTGCCGCACTTAAAAAGTTAGGTG
R-TGEV-N-565-606 GATCCACCTAACTTTTTAAGTGCGGCAAGAACAGCTTGTTCTACACTG
F-TGEV-N-736-771 AATTCGTGACAAGATTTTATGGAGCTAGAAGCAGTTCAGCCG
R-TGEV-N-736-771 GATCCGGCTGAACTGCTTCTAGCTCCATAAAATCTTGTCACG