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. 2016 Sep 30;12(9):e1005897. doi: 10.1371/journal.ppat.1005897

Fig 4. At high concentrations, the HIV-1 core-destabilizing compound PF74 induces an increase in Luciferase activity measured upon EURT assay.

Fig 4

A) Due to its well described destabilizing effect on viral cores, PF74 is expected at doses superior to 10 μM to increase the availability of EU-repRNA to the translation machinery. B) To exclude pleiotropic effects of PF74 on translation, HeLaP4 cells were transfected with an in vitro synthesized, capped mRNA coding F-Luc in the presence or absence of PF74 (at 10 μg/mL, which corresponds to 23 μM). Luciferase accumulation was measured 8 hours afterwards. C and D) HeLa P4 or MDDCs were challenged with HIV-1 virus, bearing either an X4- or an R5-tropic Env and incorporating EU-repRNA (MOI equivalent of 0.5, after exo-RT normalization against standards of known infectious titer) in the presence or absence of PF74 (as above), prior to cell lysis and Luciferase activity measurement. Averages and SEM of 6 independent experiments are presented (with cells obtained from different donors in the case of MDDCs). *statistically significant differences following a Student t test (p≤0.05).