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. 2016 Sep 30;12(9):e1005908. doi: 10.1371/journal.ppat.1005908

Fig 3. Typical deuterium accumulation plots used to identify monoclonal antibody binding epitopes on MntC.

Fig 3

Black symbols and lines—MntC alone, red symbols and lines—deuterium accumulation on MntC in the presence of mAB 305-78-7, green symbols and lines—deuterium accumulation on MntC in the presence of mAB 305-101-8, blue symbols and lines—deuterium accumulation on MntC in the presence of mAB 305-72-5. Peptide 240–261 (panel A) includes residues recognized by mAB 305-78-7, peptide 209–220 (panel B) includes major part of the mAB 305-101-8 epitope, peptides 52–66 and 82–100 (panels C and D) include residues recognized by mAB 305-72-5. The plots are scaled to the maximum number of deuterons that can exchange onto a given peptide. Deuterium accumulation plots for the corresponding peptides from all of the experiments are shown on every panel for comparison.