Fig 8. Phenotype after interaction with apoptotic cells.

DCs were mixed with apoptotic PBMC at a ratio of 1:4 for 24 hours. LPS was added 6 hours after the apoptotic cells, as indicated. Only SB- or PI-negative cells are shown; representative of 4 experiments. Top panel: The change in the expression of surface markers for all DCs is shown, normalized for iDCs (bold outline). Bottom panel: Same as the top panel, but instead of showing the results for all DCs, the MFI of iDC-S is normalized to 100; values above and below 100 indicate higher and lower expression, respectively, among DC-L as compared to DC-S.