Table 1. Primers sequences.
Gene | Primer forward (5´—3´) | Primer reverse (5´—3´) |
---|---|---|
GAPDH | CATGGCCTTCCGTGTTCCTA | GCGGCACGTCAGATCCA |
18S RNA | GGTCGGCGTCCCCCAACTTCT | CGTGCAGCCCCGGACATCTAA |
β2-microglobulin | AAGTATACTCACGCCACCCACCG | TTTTTTCCCGTTCTTCAGCATTTG |
Collagen-1α2 | CGCTCCCAGCCTTCACTCAGAC | CACGTGCGAGCAGGGTTCTTTC |
Collagen-3α1 | GGATGCAGCCACCTTGGTCAGT | GGCAGTCTAGTGGCTCCTCATCAC |
TGF-β1 | TACTGCCGCTTCTGCTCCCACTCC | TCGATGCGCTTCCGTTTCACCAG |
TGF-β3 | GGGGCGTCTCAAGAAGCAAAAG | GGGCCCTCTTCTTCCTCTGACTG |
TGF-βRc2 | CCGGGGCATCGCTCATCTC | CACACAGGCAACAGGTCAAGTCGT |
Tissue inhibitor metalloproteinase-1 (TIMP-1) | GCTAAAAGGATTCAAGGCTGTGGG | CTCTTCACTGCGGTTCTGGGACTT |
TIMP-2 | CCTCTGGATGGACTGGGTCAC | CGGGTCCTCGATGTCAAGAAACTC |
Matrix metalloproteinase-1a (MMP-1a) | TGCCAAAAGCAGTTGTGGAAGATG | GTGGAAGGAGAGCACAATATCGCC |
MMP-8 | GCCACGATGGTTGCAGAGAAGC | CACTCCACATCGAGGCATTTCCA |
MMP-12 | GGGCTAGAAGCAACTGGGCAACTG | CCATCTTGACCTCTGGGGCACTG |
MMP-13 | CCACAGTTGACAGGCTCCGAGAA | GTATTCACCCACATCAGGCACTCC |
MMP-14 | CGATGAGGGGACTGAGGAGGAGAC | CAGCAGTAGTACCGGCAGGACCAC |
MMP-15 | AACGCGGGTGACAAGGATGAGG | CCAGGCCCAGAATACAGAGCAACA |
CXCR-4 | CGATAGCCTGTGGATGGTGGTGTT | TCTGGTGGCCCTTGGAGTGTGAC |
CXCL-12 | TCCCCATTCTCCTCATCCTCATCT | CTCCCTGCAAAGCCACCGTCTAT |
T-bet | CCGGGCCTGGCGAGTTCTT | GCGGGCAATGCGTAGTCCTC |
GATA-3 | GGTCGGCCAGGCAAGATGAGAAA | TAATGGGGTGGTGGGCTGAGGAT |
RORc | CGCCGCAAGCCAGCAGTGTAAT | TCGGGACATGCCCAGAGCCA |
Ahr | GGCTGCCAGGCAGGGTTGTGA | GCTGGCCGCGGAGGAGAAAC |
FoxP3 | CCAACGCCCCAACAAGTGCTC | TCATCGCCCGGTTTCCATAGGTA |
Inducible Nitric oxide Synthase-2 (iNOS2) | GCCGCATGAGCTTGGTGTTTG | GCAGCCGGGAGTAGCCTGTGT |
Arginase-1 | CCTTGGCTTGCTTCGGAACTCA | CTTGGGAGGAGAAGGCGTTTGC |