Fig 5. Mtu1 deficiency impaired mitochondrial translation and respiratory activities in hepatocytes.

(A) Autoradiogram of mitochondrial translation in primary hepatocytes isolated from control (Flox) and hepatocyte-specific Mtu1 knockout (LKO) mice. (B) Protein levels of representative mitochondrial proteins were examined by western blotting. CI~CV indicates Complexes I ~ V. (C) Representative blue-native gel image of respiratory complexes purified from Flox and LKO mice. (D) Mitochondrial proteins incorporated in Complexes I ~ V were examined by blue-native PAGE followed by western blotting. (E) The activities of Complexes I ~ IV in the livers of Flox and LKO mice are shown. Individual complex activity was normalized to the activity of citrate synthase (CS). n = 3 each. **P < 0.01, ***P < 0.001.