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. 2016 Sep 27;9:5883–5895. doi: 10.2147/OTT.S111103

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© 2016 Ren et al. This work is published and licensed by Dove Medical Press Limited

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Western blot validations for five differential proteins.

Notes: (A and B) The results are shown as representative Western blot photograph of the five candidate biomarkers in different grades of astrocytoma. Scatter plot of EGFR (C), TIMP1 (D), TIMP1 (E), fibulin-5 (F), and fibulin-2 (G) are shown. **P<0.01, ***P<0.01. The P-value was obtained from LSD with one-way ANOVA analysis. Data are mean ± SEM.

Abbreviations: EGFR, epidermal growth factor receptor; GRB2, growth factor receptor-bound protein 2; MMP9, matrix metalloproteinase-9; TIMP, tissue inhibitor of metalloproteinase; MEK, Mitogen-activated protein kinase kinase; p-MEK, phosphorylated MEK.