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. 2016 Sep 27;9:5883–5895. doi: 10.2147/OTT.S111103

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© 2016 Ren et al. This work is published and licensed by Dove Medical Press Limited

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Fibulin-5 (A) and fibulin-2 (B) decrease glioma cells proliferation in vitro.

Notes: U251 cells were transfected with the respective plasmids. Then, relative cell numbers were determined by the MTT assay. Relative cell numbers (A and B) shown are mean ± SD of triplicate measurements and have been repeated three times with similar OD value results. The expression (C and D) of Fibulin-2 (C) or Fibulin-5 (D) was examined by Western blot assays. *P<0.05 (N=3). *P<0.05 from Bonferroni correction with or without two-way ANOVA.

Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SD, standard deviation; OD, optical density; MTT, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide.