Figure 2. SAHA promotes ROS generation in H292 cells.

Cells were treated for 24 and 48 h with 15 μM SAHA in the presence or absence of 2 mM NAC. A. Representative photomicrograph of immunofluorescence showing ROS generation in H292 cells stained with H2DCFDA after 48 h of treatment with SAHA. (Bar=40 μm and magnification 200X for all images). A positive control for ROS generation was obtained by treating the cells for 10 min with 200 μM H₂O₂ (A, bottom right and left images). In (A) the left panels show H2DCFDA-stained cells visualized by FITC filter and the right panels the corresponding images without immunofluorescence. The effect of NAC on SAHA-treated H292 cell viability was evaluated after 24 (A1) and 48 (A2) h of treatment and measured by the MTT assay. In A, A1 and A2 the results are representative of four independent experiments. *p<0.001 vs control, 15 μM SAHA and 2 mM NAC ^#p<0.001 vs control. Ctr = control.