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. 2015 Dec 19;7(20):28849–28867. doi: 10.18632/oncotarget.6680

Figure 3. SAHA induces mitochondrial depolarization in H292 cells.

Figure 3

The mitochondrial membrane potential was evaluated by fluorescence microscopy using JC-1 staining. Images were acquired using a Leica DM5000 upright fluorescence microscope (Leica Microsystems) and merges were obtained using NIH Image J 1.40 program after acquisition. The mitochondrial depolarization was observed at 24 h with 10 μM SAHA and increased with 15 μM SAHA. A, B and C panels are representative images from three independent experiments. (Bar=100 μm for all images). The insert in (C) shows the details of depolarized cells. Ctr = control.