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. 2016 Apr 9;7(20):29063–29080. doi: 10.18632/oncotarget.8666

Figure 1. Trpm8 gene encodes 5 alternate TRPM8 mRNA and 2 splice variants in human prostate.

Figure 1

(A) Genomic DNA map (not to the scale) represents the core exons of full-length TRPM8. Alternate exons are labeled with prime or second sign when are forming a cassette exon, and with “a” or “b” when they are forming the 5′ or 3′ supplemental part of a core exon respectively. Transmembrane domains and p-loop of the TRPM8 channel are aligned with their encoding exons. Full-length TRPM8 is showed in the red area (TRPM8) while 4 transmembrane domains TRPM8 isoforms are highlighted in blue (4TM-TRPM8 group) and short TRPM8 isoforms are highlighted in green (sM8 group). siRNA targeting TRPM8 are represented as small red squares aligned with the homologous TRPM8 exons. (B) Full-length amplification of TRPM8 cDNAs by PCR reveals the expression of TRPM8 mRNAs in normal prostate tissue, androgen-dependent prostate cancer cell line, LNCaP, androgen-refractory prostate cancer cell line, LNCaP C4-2b, and androgen-independent prostate cancer cell line, PC-3. TRPM8 mRNAs are referenced by their first and last exons, TRPM8 (first exon-last exon). Each PCR reaction was performed with 100 ng of cDNA. (C) Western-blot showing the detection of HA-tagged sM8 isoforms in total protein extract of HEK cells. Cells have been transfected with vector encoding sM8 mRNA for 24 h. 75 μg of total protein extract were loaded on each well. Experiments have been reproduced 3 times independently.