Figure 7. The combination of carfilzomib with vorinostat inhibits tumor growth in a human xenograft model.

(A) Nude mice (4–6 weeks old) were injected subcutaneously with 5 × 10⁶ MOLT-4 cells into the right flank. After tumors were visible, the mice received indicated doses carfilzomib with or without vorinostat as described in “Animal studies”. Tumor volume was measured every other day with calipers and calculated using the formula 0.5 × a × b² in millimeters. Results represent the Mean ± SD. (B) The body weight of mice after treatment was measured twice every week. Results represent the Mean ± SD. (C) Sections from tumor samples per group were stained with FITC-dUTP as described in materials and methods (×200). (D) Tumor samples were homogenized and lysed. The extracted proteins were probed with western blot for p-p38MAPK, p-JNK and PARP proteins. Each lane was loaded with 30 μg of protein. The β-actin was used as an internal control.