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. 2016 Apr 7;7(20):29199–29210. doi: 10.18632/oncotarget.8638

Figure 5. Dasatinib synergizes with ALK inhibitor to inhibit the viability of ALK-positive cells.

Figure 5

A. Cell viability of H2228 cells treated with/without siCRKL siRNAs in the absence and presence of 100nM crizotinib (100 nM), respectively. Data are presented as the percentage of the viable cells with treatment compared to that of untreated cells. B. Cell viability (assessed using the MTS assay) of H2228 and H3122 cells treated with crizotinib (150 nM or 300 nM), or dasatinib (from 0 to 10 μM) alone or in combination with 150 nM, or 300 nM of crizotinib. Data are presented as the percentage of viable cells compared with untreated cells. Colored scale bar: percentage of the viable cells with treatment compared to that of untreated cells. C. Effects of drug combination of dasatinib and ALK inhibitors (top panels: crizotinib; lower panels: NMS-E628) on H3122 (left two panels) and H2228 (right two panels) cell viability. The dilutions of dasatinib were made horizontally and the dilutions of ALK inhibitors vertically in a 96-well plate. Cells were incubated for 72 h before cell viability was assessed using MTS assay. Data are presented as the percentage of viable cells compared with untreated cells. Colored scale bar: percentage compared with untreated cells. Lowest FIX (Fractional Inhibitory Index) values for each combination in each cell line were calculated according to the Checkerboard Microplate Method (FIX<0.50 is considered synergy).