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. 2016 Apr 18;7(20):29333–29345. doi: 10.18632/oncotarget.8787

Figure 5. CD109 knockdown upregulated IL-8 expression through activation of TGF-β/Akt/NF-κB pathway in HUVEC.

Figure 5

(A) WB showed that CD109 knockdown significantly increased protein expression of TGF-β RI, TGF-β RII, p-Smad2, p-Akt, and p-P65 in HUVEC. (B) WB showed that SB525334 or LY294002 significantly decreased protein expression of p-Akt and p-P65 in CD109 knockdown HUVEC. (C) ELISA showed that the elevated IL-8 was significantly inhibited in HUVEC-shCD109 when pretreated with SB525334, LY294002, or IMD 0354. GAPDH served as a loading control for WB. Data shown as mean ± SD were from triplicates of three independent experiments. **P < 0.01 by t test. CM, conditioned media; NC, negative control.