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. 2016 Apr 18;7(20):29480–29491. doi: 10.18632/oncotarget.8784

Figure 3. Cytotoxicity effects of B7-H3Bi-armed ATC against different tumor cells.

Figure 3

Target cells were incubated either with B7-H3Bi-armed ATC or a combination of OKT3 and anti-B7-H3 mAb with ATC (unarmed ATC), or ATC for 18 hours, and luciferase quantitative assays were performed to determine cytotoxicity against different target cells at indicated E/T ratio. A. Bioluminescence image signal in photons per second was converted into living cell number and the cytotoxicity assays were measured at the at different E/T ratio (5:1, 10:1, and 20:1). B. Bioluminescence image of MDA-MB231-luc cell was as a representative after incubation with B7-H3Bi-armed ATC or unarmed ATC, or ATC at the indicated E/T ratio. Shown is a representative experiment of at least three, and the data for (A) are mean ± SD of triplicate determination. **P < 0.01, ***P < 0.001, B7-H3Bi-armed ATC was compared with unarmed ATC or ATCunder similar conditions.