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. 2016 Apr 18;7(20):29480–29491. doi: 10.18632/oncotarget.8784

Figure 7. The cytotoxicity effects of B7-H3Bi-armed ATC against freshly isolated tumor cell from patients.

Figure 7

A. Expression of B7-H3 on human primary breast cancer (BC) and lung cancer (LC) cell culture. Gray histogram represents cells stained with anti-human B7-H3 mAb and black histogram represents cells stained with the isotope control mAb. B. (a) Lactate dehydrogenase (LDH) activity assay was performed by B7-H3Bi-armed ATC or unarmed ATC against freshly isolated tumor cell. Supernatants of co-cultures at E/T ratio of 10:1 were harvested at 18 hour and LDH activity assay was performed to determine cytotoxicity against freshly isolated tumor cell. (b) IFN-γ, (c) TNF-α and (d) IL-2 secretion by B7-H3Bi-armed ATC against freshly isolated tumor cell. Supernatants of co-cultures at E/T ratio of 10:1 were harvested at 18 hours and analyzed for cytokine production using specific ELISA Kit. C. Target tumor cells were incubated either with B7-H3Bi-armed ATC or unarmed ATC for 18 h at E/T ratio of 10:1, and real-time photograph was taken at x 200 magnification. The data of (B) are mean ± SD of triplicate determination. Shown is a representative experiment of at least three experiments. *P < 0.05, ***P < 0.001, B7-H3Bi-armed ATC was compared with unarmed ATC under similar conditions.