Figure 6. CM-derived TREM-2⁺DCs exerted a more potent inhibitory effect on T cells proliferation.

A, B. Single cell suspension of splenocytes from C57BL/6J mice were prepared for T cells negative selection. Subsequently, TREM-2⁺DCs, and TREM-2⁻DCs derived from CM were sorted and co-cultured with CFSE-staining at a ratio of 1:5. An anti-TREM-2 mAb (10 μg/mL), an IL-10 neutralizing mAb (1 ng/mL) and Syk inhibitor (R406, 4μg/mL) were added to the co-culture system, and 72 h later, proliferation of T cells was detected by FACS and analyzed by FlowJo. The program calculated the percentage of divided cells (% divided) based on the CFSE dilution levels in dividing cells. **p<0.01 compared with other four groups.