Fig. 1.

TRAIL-induced apoptosis in the presence of chemotherapeutic agents in MDA-MB435S cells. a MDA-MB435S cells were treated with the indicated chemotherapeutics (10 μM etoposide, 10 μM cisplatin, 20 μM roscovitine, 10 μM paclitaxel, 1 μM doxorubicin, 0.5 μg/ml CHX) in combination with scFv62-TRAIL (0.1 μg/ml) for 18 h. Apoptosis was determined by Annexin V staining and flow cytometry. Asterisks indicate statistical significance (**p < 0.01; ****p < 0.0001; two-way ANOVA). MDA-MB435S cells were treated with scFv62-TRAIL (0.1 μg/ml) with different concentrations of etoposide (b), doxorubicin (c), and roscovitine (d) and analyzed with Annexin V staining and flow cytometry. e Data from b–d are represented as percent apoptosis in the presence of scFv62-TRAIL versus apoptosis induction in the absence of the construct. The synergistic effect was comparable for all drugs and linearly correlated to the concentration of the drug