Fig. 2.

Analysis of scFv62-TRAIL induced apoptosis. a Association and dissociation constants between the immunogen (h1x) and the scFv62-TRAIL construct were determined by SPR. Sensograms were recorded for binding of the indicated concentrations of scFv62-TRAIL (analyte) to immobilized h1x (ligand). b Apoptosis induction by scFv62-TRAIL requires both the antibody part and active TRAIL. MDA-MB435S cells were treated with scFv62-TRAIL that had been pre-incubated with excess antigen (1:50) or with neutralizing anti-TRAIL antibody (1:10) for 1 h. Apoptosis induction was analyzed with Annexin V staining and flow cytometry 18 h after the addition 0.1 µg/ml scFv62-TRAIL and 0.5 μg/ml CHX. Student’s t test, **p < 0.01, ***p < 0.001