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. 2016 Jun 27;45(7):709–719. doi: 10.1007/s00249-016-1149-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — a Expression of TRAIL receptors mRNA in MDA-MB435S. Quantitative real-time PCR on cDNA from the cell line revealed abundant message for TRAIL-R1 and TRAIL-R2, the latter being more abundant. b Both TRAIL-R1 and TRAIL-R2 can mediate the effect to scFv62-TRAIL. MDA-MB435S cells were treated with siRNA against, TRAIL-R1, TRAIL-R2, or both apoptotic receptors in combination (30 nM); 24 h later cells were treated with scFv62-TRAIL (0.1 μg/ml) in combination with 0.5 μg/ml CHX for 18 h. Apoptosis induction was measured with Annexin V staining and flow cytometry. Only when both receptors were knocked one there was a significant reduction in apoptosis induction