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The domain deletion mutants were constructed as described in Materials and Methods. The protein expressions of hFXRΔLBD (A), hFXRΔHinge/LBD (B), hFXRΔAF1 (C), and hFXR DBD (D). COS-1 cells were transfected with each plasmid for 24 hours and then were treated with 10μM MG132 for 3 hours. The protein extracts were subjected to Western blot analysis. For detection, the hFXR DBD was expressed as C-terminal V5-tagged protein.
