Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
COS-1 cells were transfected with full-length form of hFXR S154D (A) or its ΔLBD mutant (B) along with ubiquitin for 24 hours and then were treated with 10μM MG132 for 6 hours. The protein extracts were immunoprecipitated with anti-FXR antibody followed by Western blotting with anti-ubiquitin antibody.
