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The plasmids which express the hRXRα AF1 and hFXRΔAF1/LBD mutants or hFXR AF1 and hRXRαΔAF1/LBD were transfected into COS-1 cells for 24 hours. The protein expressions were examined after 10μM MG132 treatment for 3 hours using anti-RXRα or anti-FXR for each chimera molecule.
