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Protein concentration of LβT2 cell lysates was determined and equal amounts examined by Western blotting. Membranes were probed with anti-PAD1, anti-PAD2, anti-PAD3, and anti-PAD4 antibodies or anti-β-actin for a loading control. Positive controls (+) were generated by transient overexpression of PAD1–PAD4 plasmids in LβT2 cells.
