Figure 1.

A, Anatomical representation of regions extracted via micropunch (obtained by approaching the regions of interest caudally and punching rostrally) and used for gene expression analysis. For each animal, 1 pair of bilateral anterodorsal hippocampal punches (1, unshaded) and 1 pair of bilateral caudoventral hippocampal punches (2, unshaded) were made, each 0.5 mm in diameter and 1.00 mm in depth. All 4 punches were combined, collectively comprising the whole hippocampus. Hypothalamic tissue consisted of 2 sequential punches (1.25 mm in diameter and 1.00 mm in depth): 1 anteromedial (3, shaded) and 1 caudomedial (4, shaded). B, Sample sizes for RNA-seq and qRT-PCR.