Skip to main content
. 2016 Aug 29;157(10):3958–3971. doi: 10.1210/en.2016-1472

Figure 4.

Figure 4.

A, Total GH recovered in both eGFP and tdTomato fractions showed that GH proteins are reduced significantly in both male and female somatotrope Lepr-null mutants (Mut) compared with controls (Con). *, Lowest values (ANOVA/Newman-Keuls, P = .008; n = 5/group). B, The percentage of the total GH recovered in eGFP fractions is similar in all experimental groups (ANOVA), ranging from 74% to 83% of the total GH. C, Reduced GH protein levels in eGFP fractions from the Lepr-null somatotrope fractions (Mut) compared with controls. *, Lowest values (ANOVA/Newman-Keuls, P = .001; n = 5/group). D, No changes in GH proteins recovered from tdTomato fractions when comparing males, females, or controls and mutants. E, Gh mRNA is highest in eGFP fractions from females, significantly different from mutants and both groups of males (line indicates lowest values, ANOVA/Newman-Keuls, P < .0001, n = 5–8/group). F, Gh mRNA is low in tdTomato fractions. An ANOVA shows no significant difference between males, females, or controls and mutants.