A, Distribution of TSH across the two fractions (eGFP and tdTomato) shows that TSH is lowest in eGFP fractions from mutant females or both groups of males. There are no differences when the remaining groups are compared. Capped bar, Lowest values, different from all others (ANOVA/Newman-Keuls, P = .0037; n = 4–5). *, Significantly different from control eGFP fraction (Student's t test, P = .047). B, Analysis of Tsh mRNA distribution in females shows significantly higher expression in tdTomato fractions and no differences between controls and mutants. Capped line, Lowest values by ANOVA/Newman-Keuls (P < .0001; n = 10–15). Con, control; Mut, somatotrope Lepr-null mutant.