Figure 4.

Placental LAT2 protein was quantified by Western blot analysis at GD19 in MiMCR vs CON (A), MoMCR vs CON (B), and glut1 (+/−) vs wt (+/+) (C). Upper panels demonstrate representative Western blots depicting the LAT2 protein bands with the corresponding vinculin protein bands, which served as the internal control in CON and CR GD19 placentas. Lower panels demonstrate the densitometric quantification of LAT2/vinculin protein, depicted as a percentage of CON/wt values. *, P = .02 vs CON. CR, respective maternal calorie restriction.