Figure 4.

In vitro pancreatic islet insulin secretion. Pancreatic islets were isolated from CON (white bars, n = 10) and IUGR (black bars, n = 16) fetuses and incubated overnight with or without additional amino acids (2× or 6×) or HGF (100 ng/mL). A, Insulin content was lower in IUGR islets than CON islets (P < .0005). CON islet insulin content increased with increasing overnight amino acid supplementation (*, relative to basal conditions, P < .005), an effect not seen in IUGR islets. B, Overnight amino acid supplementation had no effect on IUGR islet glucose and potassium-stimulated insulin secretion. However, CON islets had a dose-response increase in glucose and potassium-stimulated insulin secretion after overnight incubation with supplemental amino acids (P < .05); *, significantly more fractional insulin release from IUGR islets compared with CON islets incubated in the same conditions (P < .05). C, Overnight incubation with HGF resulted in higher insulin contents of both CON and IUGR islets (P < .05). D, Overnight incubation in HGF had no effect on CON or IUGR islet glucose or potassium-stimulated insulin secretion. Data are presented as mean ± SE, and statistics are by mixed models ANOVA.