Figure 5.

Large vessel endothelial cell HGF mRNA is increased with in vitro supplemental amino acids, leucine, and VEGFA. Large vessel endothelial cells from normal fetal sheep (n = 3) were incubated for 72 hours in basal conditions or with indicated supplementation (amino acids [2× or 6×], leucine [2.4 mmol/L], VEGFA [50 ng/mL]). HGF mRNA was then measured and normalized to basal conditions. For each incubation condition, cells derived from all 3 animals were tested in replicates of 1–3; *, significant difference from basal conditions (P < .005). Data are presented as mean ± SE, and statistics are by mixed models ANOVA after log transformation.