Figure 4.

Effects of rhCXCL-12 on the induction by bTSH and M22 of IL-6 in fibrocytes and GD-OF. A, Graded concentrations of rhCXCL-12 were added to confluent fibrocyte cultures for 6 hours, monolayers harvested, and RNA extracted and subjected to real-time PCR for IL-6 mRNA. rhCXCL-12 (50 ng/mL), bTSH (5 mIU/mL), and M22 (1 μg/mL) were added for 6 hours in the combinations indicated along the abscissas to (B) fibrocytes and (C) GD-OF. D, Parental GD-OFs were subjected to cytometric cell sorting into pure CD34⁺ OF and CD34⁻ OF. These were then treated as in B and C with the indicated combinations for 6 hours, and then RNA was processed for real-time PCR targeting IL-6 mRNA. Data are expressed as the mean ± SD of 3 independent replicates.