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The effects of AMD3100 on the induction of IL-6 mRNA by rhCXCL-12 and bTSH. Confluent cultures of fibrocytes were treated with AMD3100 (5μM), bTSH (5 mIU/mL), and rhCXCL-12 (50 ng/mL) in the combinations indicated for 6 hours. RNA was extracted from monolayers and processed for real-time PCR for IL-6 mRNA. Data are expressed as the mean ± SD of 3 independent replicates.
