Table II.
1H- and 13C-NMR chemical shifts and selected inter-residue (HMBC) connectivities obtained for the anomeric protons of L919/B from Lactobacillus casei LOCK 0919
| Sugar residue | Chemical shifts 1H, 13C (ppm) | Selected connectivities (ppm) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| H1 | H2 | H3 | H4 | H5 | H6 | H6′ | CH3CO | NOESY | HMBC | ||
| C1 | C2 | C3 | C4 | C5 | C6 | ||||||
| A | →6)-β-D-ManpNAc-(1→ | 5.02 | 4.43 | 3.74 | 3.46 | 3.26 | 4.01 | 3.72 | 1.93 | A1-B4 (4.26) | A1-A2 (intra) |
| 98.6 | 53.0 | 71.8 | 66.8 | 75.6 | 69.4 | 22.1 | A1-B4 (75.5) | ||||
| B | →3,4)-α-D-Galp-(1→ | 4.81 | 3.80 | 3.95 | 4.26 | 4.06 | 3.68 | B1-D4 (3.52) | B1-B5 (intra) | ||
| B1-B5 (4.06) | |||||||||||
| C | →3)-β-D-GalpNAc-(1→ | 98.2 | 67.9 | 77.4 | 75.5 | 69.9 | 61.0 | B1- B6 (3.68) | |||
| 4.65 | 3.97 | 3.77 | 4.02 | 3.56 | 3.70 | 1.95 | C1-B3 (3.95) | C1-B3 (77.4) | |||
| 21.9 | |||||||||||
| D | →4,6)-β-D-Glcp-(1→ | 103.3 | 51.5 | 80.3 | 67.9 | 74.7 | 60.3 | ||||
| 4.46 | 3.28 | 3.36 | 3.52 | 3.39 | 3.95 | 3.65 | D1-C3 (3.77) | D1-C3 (80.3) | |||
| 104.6 | 72.8 | 75.7 | 73.8 | 75.3 | 64.8 | ||||||
| E | β-D-Glcp-(1→ | 4.37 | 3.26 | 3.37 | 3.27 | 3.38 | 3.63 | 3.80 | E1-A6 (4.01 and 3.72) | E1-A6 (69.4) | |
| 102.5 | 72.9 | 75.5 | 69.2 | 75.9 | 60.6 | ||||||
| P 2.6 ppm | P-D6 (64,8) | ||||||||||
Spectra were obtained for 2H2O solutions at 25°C, with acetone (δH 2.225, δC 31.05 ppm) used as an internal reference.