Fig. 2.
T cells from PSA-treated DC-cKO protect recipients from asthma. DC-cKO and WT mice were treated with PSA over 12 d, and CD4+ T cells were harvested on day 15. Recipients, all WT, received 2 × 106 each of OT2 T cells and T cells from PSA-treated DC-cKO or WT mice, then challenged intranasally for 1 week. On day 7, lungs were lavaged and analyzed for cellularity. The transfer of PSA-exposed T cells from both mice reduced leukocyte (A), lymphocyte (B) and macrophage (C) infiltration equally. Neutrophil (D) infiltration was essentially unchanged in positive and negative controls, although DC-cKO transfer did show a reduction compared to WT. Statistical comparisons are made between WT and DC-cKO T cell transfers, generally showing no difference (P > 0.05).