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Splenocytes show varied Mgat2 ablation. DC-cKO (cKO) Spls were harvested and single cells stained with PHA-L and antibodies against CD11c (A), CD11b (B), CD4 (C), CD8 (D) or CD19 (E), plotted with side-scatter and compared to WT splenocytes (F). We found that 23% of CD11c+ cells, 31.6% CD11b+, 11.7% CD4+, 6.6% of CD8+ and 21.0% of CD19+ B cells lacked complex N-glycans (PHA-L low) in DC-cKO mice compared to <3% in each WT population (n = 3; P < 0.0001 compared to WT cells).
