Table 3.
Relationship between monocyte bioenergetics with skeletal and cardiac muscle bioenergetics. Pearson correlations and 95% confidence intervals of monocyte respiratory parameters with bioenergetics from permeabilized skeletal muscle fibers and isolated mitochondria from vastus lateralis and cardiac tissue.
| Basal (95% CI) | MAX (95% CI) | Reserve (95% CI) | |
|---|---|---|---|
| Permeabilized fibers: | |||
| Basal | −0.43 (−0.74 to 0.03) | −0.42 (−0.74 to 0.03) | −0.33 (−0.65 to 0.17) |
| Complex I OXPHOS | 0.74 (0.42–0.91) | 0.73 (0.33–0.91) | 0.60 (0.03–0.91) |
| Complex I+II OXPHOS | 0.79 (0.49–0.96) | 0.75 (0.38–0.97) | 0.57 (0.06–0.92) |
| MAX | 0.09 (−0.51 to 0.73) | −0.01 (−0.59 to 0.70) | −0.20 (−0.67 to 0.57) |
| Left vastus CII: | |||
| State 3 | −0.24 (−0.73 to 0.55) | −0.24 (−0.79 to 0.63) | −0.23 (−0.86 to 0.67) |
| MAX | −0.20 (−0.73 to 0.55) | −0.18 (−0.76 to 0.56) | −0.13 (−0.79 to 0.62) |
| RCR-A | 0.87 (0.58–0.96) | 0.83 (0.49–0.97) | 0.69 (0.22–0.98) |
| RCR-F | 0.71 (0.30–0.91) | 0.70 (0.35–0.89) | 0.62 (0.10–0.89) |
| Cardiac CII: | |||
| State 3 | 0.06 (−0.36 to 0.68) | 0.09 (−0.40 to 0.64) | 0.14 (−0.45 to 0.64) |
| MAX | 0.16 (−0.29 to 0.85) | 0.19 (−0.32 to 0.82) | 0.22 (−0.43 to 0.74) |
| RCR-A | 0.49 (0.14–0.81) | 0.55 (0.22–0.86) | 0.58 (0.28–0.87) |
| RCR-F | 0.52 (−0.21 to 0.98) | 0.50 (−0.18 to 0.96) | 0.41 (−0.11 to 0.89) |
CII=complex 2, FCCP=carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone, MAX=FCCP-linked respiration, OXPHOS=oxidative phosphorylation capacity, RCR=respiratory control ratio. Bold type = p-value ≤ 0.05.
Respiration is measured as oxygen consumption rate (pmol/min/250,000 cells [monocytes], pmol/min/mg dry weight [permeabilized fibers], pmol/min/5 µg mitochondrial protein [left vastus and cardiac tissues]).
Reserve capacity is calculated as the difference between MAX and Basal respiration.
RCRs are calculated as ADP-stimulated State 3 respiration divided by oligomycin-linked respiration (RCR-A) and by MAX respiration divided by oligomycin-linked respiration (RCR-F).
In permeabilized muscle fiber preparations, basal respiration was measured with no added substrates, leak was measured with malate added, complex I OXPHOS capacity was measured using pyruvate, glutamate, and malate with a saturating amount of ADP. Complex I+II OXPHOS capacity was measured using complex I substrates with succinate added. MAX respiration was measured using complex I+II substrates with FCCP titrations added. Non-mitochondrial respiration was measured with MAX substrates with antimycin-A and rotenone substrates added.
In isolated mitochondrial preparations, CII respiration was measured using succinate and rotenone as substrates and using ADP, oligomycin, and FCCP injections to measure State 3, oligomycin, and MAX respiration, respectively.
N=11–12.