Table 5.
Partial correlations between monocyte and platelet bioenergetics with permeabilized skeletal muscle bioenergetics. Partial correlations and 95% confidence intervals of monocyte and platelet respiratory parameters with bioenergetics from permeabilized skeletal muscle fibers left vastus lateralis, controlling for age, weight, and insulin status.
|
Partial (age) |
Partial (weight) |
Partial (HOMA-IR) |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Permeabilized fibers: | Basal (95% CI) | MAX (95% CI) | Reserve (95% CI) | Basal (95% CI) | MAX (95% CI) | Reserve (95% CI) | Basal (95% CI) | MAX (95% CI) | Reserve (95% CI) |
| Monocytes: | |||||||||
| Basal | −0.55 (−0.83 to −0.17) | −0.49 (−0.82 to 0.03) | −0.36 (−0.79 to 0.42) | −0.47 (−0.79 to −0.14) | −0.46 (−0.79 to −0.09) | −0.38 (−0.78 to 0.22) | −0.51 (−0.85 to −0.04) | −0.50 (−0.83 to 0.12) | −0.43 (−0.76 to 0.30) |
| Complex I OXPHOS | 0.64 (0.13–0.89) | 0.63 (0.13–0.90) | 0.56 (−0.11 to 0.90) | 0.76 (0.26–0.92) | 0.75 (0.26–0.92) | 0.63 (−0.03 to 0.91) | 0.74 (0.16–0.95) | 0.73 (0.13–0.95) | 0.61 (−0.15 to 0.91) |
| Complex I+II OXPHOS | 0.71 (0.26–0.96) | 0.67 (0.02–0.97) | 0.52 (−0.30 to 0.95) | 0.79 (−0.30 to 0.97) | 0.75 (−0.28 to 0.98) | 0.57 (−0.42 to 0.93) | 0.79 (0.39–0.98) | 0.75 (0.34–0.97) | 0.57 (−0.03 to 0.91) |
| MAX | −0.19 (−0.72 to 0.56) | −0.27 (−0.77 to 0.52) | −0.37 (−0.81 to 0.40) | 0.21 (−0.63 to 0.86) | 0.12 (−0.85 to 0.69) | −0.07 (−0.77 to 0.66) | 0.06 (−0.57 to 0.76) | −0.05 (−0.64−0.68) | −0.25 (−0.81−0.47) |
| Platelets: | |||||||||
| Basal | −0.25 (−0.72 to 0.34) | −0.04 (−0.59 to 0.51) | 0.21 (−0.40 to 0.69) | −0.07 (−0.71 to 0.32) | 0.07 (−0.58 to 0.47) | 0.25 (−0.40 to 0.63) | −0.11 (−0.51 to 0.35) | 0.06 (−0.42 to 0.48) | 0.25 (−0.43 to 0.70) |
| Complex I OXPHOS | 0.18 (−0.40 to 0.69) | 0.34 (−0.22 to 0.78) | 0.08 (−0.14 to 0.79) | 0.19 (−0.39 to 0.60) | 0.34 (−0.20 to 0.66) | 0.46 (−0.09 to 0.79) | 0.14 (−0.48 to 0.60) | 0.30 (−0.27 to 0.65) | 0.43 (−0.15 to 0.74) |
| Complex I+II OXPHOS | 0.34 (−0.16 to 0.83) | 0.52 (−0.11 to 0.84) | 0.60 (0.13–0.84) | 0.34 (−0.27 to 0.81) | 0.52 (0.11–0.78) | 0.62 (0.19–0.84) | 0.31 (−0.31 to 0.79) | 0.50 (0.01–0.79) | 0.60 (0.19–0.82) |
| MAX | 0.17 (−0.36 to 0.68) | 0.40 (−0.05 to 0.76) | 0.56 (0.12–0.88) | 0.25 (−0.36 to 0.67) | 0.43 (−0.05 to 0.73) | 0.57 (0.15–0.83) | 0.25 (−0.29 to 0.67) | 0.46 (−0.02 to 0.75) | 0.59 (0.18–0.85) |
FCCP=carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone, HOMA-IR=homeostatic model of insulin resistance, MAX=FCCP-linked respiration, OXPHOS=oxidative phosphorylation capacity. Bold type = p-value ≤ 0.05.
Respiration is measured as oxygen consumption rate (pmol/min/mg protein [platelets], pmol/min/250,000 cells [monocytes], and pmol/min/mg dry weight [permeabilized fibers]).
Reserve capacity is calculated as the difference between MAX and Basal respiration.
In permeabilized muscle fiber preparations, basal respiration was measured with no added substrates, leak was measured with malate added, complex I OXPHOS capacity was measured using pyruvate, glutamate, and malate with a saturating amount of ADP. Complex I+II OXPHOS capacity was measured using complex I substrates with succinate added. MAX respiration was measured using complex I+II substrates with FCCP titrations added. Non-mitochondrial respiration was measured with MAX with antimycin-A and rotenone substrates added.
Monocytes: N=12. Platelets: N=17.