Table 6.
Partial correlations between monocyte bioenergetics with skeletal and cardiac muscle bioenergetics. Partial correlations and 95% confidence intervals of monocyte respiratory parameters with bioenergetics from isolated mitochondria from left vastus lateralis and cardiac tissue, controlling for age, weight, and insulin status.
|
Partial (age) |
Partial (weight) |
Partial (HOMA-IR) |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Basal (95% CI) | MAX (95% CI) | Reserve (95% CI) | Basal (95% CI) | MAX (95% CI) | Reserve (95% CI) | Basal (95% CI) | MAX (95% CI) | Reserve (95% CI) | |
| Left vastus CII: | |||||||||
| State 3 | −0.45 (−0.94 to 0.85) | −0.43 (−0.94 to 0.92) | −0.34 (−0.94 to 0.85) | −0.19 (−0.81 to 0.29) | −0.19 (−0.80 to 0.22) | −0.17 (−0.74 to 0.15) | −0.37 (−0.87 to 0.68) | −0.39 (−0.87 to 0.59) | −0.39 (−0.91 to 0.54) |
| MAX | −0.22 (−0.90 to 0.90) | −0.19 (−0.87 to 0.93) | −0.13 (−0.88 to 0.92) | −0.18 (−0.78 to 0.37) | −0.16 (−0.76 to 0.26) | −0.11 (−0.68 to 0.24) | −0.37 (−0.88 to 0.71) | −0.37 (−0.91 to 0.76) | −0.33 (−0.95 to 0.74) |
| RCR-A | 0.80 (0.32–0.97) | 0.78 (0.29–0.98) | 0.65 (0.03–0.97) | 0.86 (0.37–0.97) | 0.83 (0.36–0.97) | 0.67 (−0.11 to 0.98) | 0.89 (0.57–0.98) | 0.84 (0.45–0.98) | 0.65 (0.02–0.99) |
| RCR-F | 0.74 (0.33–0.93) | 0.72 (0.28–0.95) | 0.61 (−0.15 to 0.95) | 0.68 (0.14–0.92) | 0.67 (0.09–0.89) | 0.58 (−0.25 to 0.84) | 0.73 (−0.36 to 0.99) | 0.71 (−0.15 to 0.99) | 0.59 (0.04–0.96) |
| Cardiac CII: | |||||||||
| State 3 | 0.06 (−0.55 to 0.80) | 0.10 (−0.55 to 0.74) | 0.14 (−0.63 to 0.69) | 0.13 (−0.42 to 0.82) | 0.18 (−0.47 to 0.89) | 0.26 (−0.51 to 0.82) | 0.11 (−0.47 to 0.76) | 0.16 (−0.50 to 0.82) | 0.22 (−0.67 to 0.85) |
| MAX | 0.17 (−0.44 to 0.90) | 0.20 (−0.43 to 0.87) | 0.22 (−0.50 to 0.81) | 0.22 (−0.34 to 0.83) | 0.26 (−0.46 to 0.88) | 0.31 (−0.60 to 0.89) | 0.24 (−0.26 to 0.78) | 0.29 (−0.35 to 0.85) | 0.33 (−0.61 to 0.90) |
| RCR-A | 0.32 (−0.40 to 0.90) | 0.42 (−0.32 to 0.91) | 0.53 (−0.05 to 0.87) | 0.53 (−0.33 to 0.91) | 0.61 (−0.10 to 0.94) | 0.66 (0.27–0.97) | 0.45 (−0.27 to 0.93) | 0.51 (−0.18 to 0.98) | 0.54 (−0.53 to 0.99) |
| RCR-F | 0.36 (−0.77−0.93) | 0.36 (−0.78 to 0.94) | 0.33 (−0.77 to 0.92) | 0.57 (−0.44 to 0.96) | 0.57 (−0.51 to 0.97) | 0.48 (−0.39 to 0.95) | 0.52 (−0.01 to 0.96) | 0.51 (−0.26 to 0.97) | 0.42 (−0.37 to 0.92) |
CII=complex 2, FCCP=carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone, HOMA-IR=homeostatic model of insulin resistance, MAX=FCCP-linked respiration, OXPHOS=oxidative phosphorylation capacity, RCR=respiratory control ratio. Bold type = p-value ≤ 0.05.
Respiration is measured as oxygen consumption rate (pmol/min/250,000 cells [monocytes] and pmol/min/5 µg mitochondrial protein [left vastus and cardiac tissues]).
Reserve capacity is calculated as the difference between MAX and Basal respiration.
RCRs are calculated as ADP-stimulated State 3 respiration divided by oligomycin-linked respiration (RCR-A) and by MAX respiration divided by oligomycin-linked respiration (RCR-F).
In isolated mitochondrial preparations, CII respiration was measured using succinate and rotenone as substrates and using ADP, oligomycin, and FCCP injections to measure State 3, oligomycin, and MAX respiration, respectively.
N=11–12.