Table 7.
Partial correlations between platelet bioenergetics with skeletal and cardiac muscle bioenergetics. Partial correlations and 95% confidence intervals of platelet respiratory parameters with bioenergetics from isolated mitochondria from left vastus lateralis and cardiac tissue, controlling for age, weight, and insulin status.
|
Partial (age) |
Partial (weight) |
Partial (HOMA-IR) |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Basal (95% CI) | MAX (95% CI) | Reserve (95% CI) | Basal (95% CI) | MAX (95% CI) | Reserve (95% CI) | Basal (95% CI) | MAX (95% CI) | Reserve (95% CI) | |
| Left vastus CII: | |||||||||
| State 3 | 0.05 (−0.52 to 0.67) | 0.34 (−0.02 to 0.69) | 0.59 (0.20–0.85) | 0.03 (−0.68 to 0.55) | 0.30 (−0.32 to 0.62) | 0.57 (0.07–0.80) | −0.02 (−0.54 to 0.59) | 0.28 (−0.13 to 0.66) | 0.56 (0.10–0.84) |
| MAX | 0.26 (−0.32 to 0.75) | 0.51 (0.11–0.81) | 0.67 (0.29–0.92) | 0.10 (−0.61 to 0.59) | 0.35 (−0.24 to 0.67) | 0.58 (0.12–0.84) | 0.02 (−0.52 to 0.62) | 0.30 (−0.18 to 0.74) | 0.55 (0.05–0.90) |
| RCR-A | 0.23 (−0.27 to 0.83) | 0.42 (0.04–0.81) | 0.53 (0.03–0.83) | 0.43 (−0.19 to 0.85) | 0.58 (0.18–0.86) | 0.63 (0.21–0.89) | 0.29 (−0.31 to 0.84) | 0.44 (0.00–0.80) | 0.51 (−0.05 to 0.84) |
| RCR-F | 0.56 (0.08–0.93) | 0.70 (0.39–0.91) | 0.71 (0.37–0.92) | 0.50 (−0.09 to 0.93) | 0.67 (0.29–0.91) | 0.73 (0.45–0.91) | 0.36 (−0.25 to 0.91) | 0.55 (0.08–0.86) | 0.64 (0.25–0.86) |
| Cardiac CII: | |||||||||
| State 3 | −0.27 (−0.68 to 0.28) | −0.05 (−0.53 to 0.44) | 0.21 (−0.25 to 0.65) | −0.26 (−0.60 to 0.11) | −0.08 (−0.46 to 0.31) | 0.18 (−0.21 to 0.53) | −0.24 (−0.70 to 0.40) | −0.04 (−0.58 to 0.54) | 0.23 (−0.30 to 0.70) |
| MAX | −0.13 (−0.61 to 042) | 0.06 (−0.48 to 0.55) | 0.26 (−0.23 to 067) | −0.16 (−0.53 to 0.21) | 0.01 (−0.41 to 0.37) | 0.22 (−0.21 to 0.58) | −0.10 (−0.60 to 0.40) | 0.08 (−0.48 to 0.55) | 0.30 (−0.24 to 0.71) |
| RCR-A | 0.16 (−0.49 to 0.60) | 0.29 (−0.41 to 0.71) | 0.34 (−0.28 to 0.77) | 0.35 (−0.16 to 0.71) | 0.43 (−0.13 to 0.84) | 0.42 (0.01–0.77) | 0.29 (−0.36 to 0.74) | 0.37 (−0.27 to 0.84) | 0.37 (−0.15 to 0.79) |
| RCR-F | 0.46 (−0.01 to 0.78) | 0.49 (−0.02 to 0.80) | 0.39 (−0.10 to 0.73) | 0.56 (−0.10 to 0.90) | 0.58 (−0.09 to 0.87) | 0.46 (−0.07 to 0.80) | 0.60 (0.21–0.84) | 0.62 (0.24–0.88) | 0.48 (0.08–0.80) |
CII=complex 2, FCCP=carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone, HOMA-IR=homeostatic model of insulin resistance, MAX=FCCP-linked respiration, OXPHOS=oxidative phosphorylation capacity, RCR=respiratory control ratio. Bold type = p-value ≤ 0.05.
Respiration is measured as oxygen consumption rate (pmol/min/mg protein [platelets] and pmol/min/5 µg mitochondrial protein [left vastus and cardiac tissues]).
Reserve capacity is calculated as the difference between MAX and Basal respiration.
RCRs are calculated as ADP-stimulated State 3 respiration divided by oligomycin-linked respiration (RCR-A) and by MAX respiration divided by oligomycin-linked respiration (RCR-F).
In isolated mitochondrial preparations, CII respiration was measured using succinate and rotenone as substrates and using ADP, oligomycin, and FCCP injections to measure State 3, oligomycin, and MAX respiration, respectively.
N=16.