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. 2016 Oct 1;11:45. doi: 10.1186/s13020-016-0116-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of trans-FA on cell-cycle progression of H1299 cells. H1299 cells were treated with the indicated doses, 0.03, 0.06, 0.15, 0.3 and 0.6 mM of trans-FA for 48 h respectively. a An accumulation of G₀/G₁ population in trans-FA-treated H1299 cells and vehicle controls at 48 h. b The quantification analysis. Data are presented as mean ± SD (n = 3). Different letter notations indicate the statistical significance between control and trans-FA treatment groups (a no significance; a vs b and a vs c, statistically significant with P < 0.05 and 0.001, respectively)