Fig. 5.

Functional assessment of in vitro shRNA-mediated inhibition of Galm and Asns expression in 3T3-L1 adipocytes. a Asns mRNA expression levels in anti-Asns shRNA-treated cells expressed as a percentage of control 3T3-L1 cells. b Galm mRNA expression levels in anti-Galm shRNA-treated cells expressed as a percentage of control 3T3-L1 cells. c Intracellular lipid content of differentiated 3T3-L1 cells was measured by absorbance at 590 nm after Oil Red-O staining. d Glucose uptake was evaluated by measurement of radiolabeled 2-deoxyglucose present in 3T3-L1 cells following insulin stimulation and normalized to protein level. Shown data represent means ± standard error of the mean. Mann–Whitney tests were performed: *P < 0.05, **P < 0.01, and ***P < 0.001 significantly different to control; and ⁺⁺⁺ P < 0.001 significantly different to Galm-deficient cells