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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Nat Med. 2016 Feb 29;22(4):369–378. doi: 10.1038/nm.4053

Figure 5. Cell cycle progression activity and E2F1 expression are inversely associated with AR activity.

Figure 5

(a) CCP scores are inversely associated with AR activity scores as determined by the expression levels of 21 AR-regulated genes in 171 tumors (r = −0.36, P < 0.001).

(b) Inverse association between CCP scores and AR activity scores was also observed in the Grasso et al. dataset of 35 CRPC tumors (r = −0.42, P = 0.01).

(c) AR activity is inversely associated with E2F1 transcript expression in 171 tumors (r = −0.43, P < 0.001).

(d) AR expression level influences cellular responses to the AR ligand R1881. Representative Western blot (n = 2) of AR and GAPDH protein levels in wild type LNCaP cells and LNCaP-AR cells engineered to overexpress AR (rAR) (left panel). LNCaP and LNCaP-AR cell growth measured 72 h after exposure to the indicated R1881 concentration, n = 4 in each group (right panel).