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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Dev Biol. 2016 Aug 18;418(1):40–54. doi: 10.1016/j.ydbio.2016.08.018

Figure 1. FoxO regulates class IV dendrite morphology.

Figure 1

(A, B, H, I) Representative z-projections of class IV ddaC neurons marked with mCD8-GFP driven by 477-GAL4 at the indicated larval ages and backgrounds. (C) Quantification of dendrite branch point numbers at 72 h AEL in control animals: 390.6 ± 32.8, n=8 cells; foxOΔ94 animals: 209.5 ± 30.7, n=6 cells. (D) Quantification of dendrite length at 72 h AEL in control animals: 6.78 ± 0.48 mm, n=8; foxOΔ94 animals: 4.91 ± 0.50 mm, n=6 cells. (E, F, L, M) Representative analysis of internal coverage of ddaC cells with 250 μm2 squares of the indicated ages and backgrounds. Green squares mark areas covered by the dendritic arbor and soma, while magenta squares mark areas not covered. (G) Quantification of the proportion of squares not covered by the dendrite and soma at 72 h AEL in control animals: 0.10 ± 0.01, n=8 cells; foxOΔ94 animals: 0.21 ± 0.01, n=6 cells. (J) Quantification of dendrite branch point numbers at 120 h AEL in control animals: 678.5 ± 26.2, n=6 cells; foxOΔ94 animals: 453.3 ± 35.0, n=8 cells. (K) Quantification of dendrite length at 120 h AEL in control animals: 15.48 ± 0.44 mm, n=6 cells; foxOΔ94 animals: 11.04 ± 0.54 mm, n=8 cells. (N) Quantification of the proportion of squares not covered by the dendrite and soma at 120 h AEL in control animals: 0.19 ± 0.02, n=6 cells; foxOΔ94 animals: 0.33 ± 0.01, n=8 cells. Scale bars: 50 μm. Error bars are mean ± s.e.m., *, p<0.05, **, p<0.01, ***, p<0.001.