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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Dev Biol. 2016 Aug 18;418(1):40–54. doi: 10.1016/j.ydbio.2016.08.018

Figure 3. FoxO is expressed in da neurons and regulates class I–III dendrite morphology.

Figure 3

(A–D) FoxO staining (green) of FoxO positive controls and foxOΔ94 class I–IV cells, counterstained for HRP (red). Scale bar: 5 μm. (E, F, I, J, M, N) Representative z-projections of class I and II cells of the indicated cell type and backgrounds, marked with mCD8-GFP driven by C161-GAL4. (G) Quantification of branch point numbers in class I ddaE cells in control animals: 20.6 ± 0.6, n=10 cells; foxOΔ94 animals: 16.5 ± 0.6, n=10 cells. (H) Quantification of dendrite length in class I ddaE in control animals: 1.55 ± 0.04 mm, n=10 cells; foxOΔ94 animals: 1.31 ± 0.06 mm, n=10 cells. (K) Quantification of branch point numbers in class I vpda cells in control animals: 36.6 ± 1.3, n=7 cells; foxOΔ94 animals: 24.8 ± 1.0, n=8 cells. (L) Quantification of dendrite length in class I vpda in control animals: 1.62 ± 0.07 mm, n=7 cells; foxOΔ94 animals: 1.32 ± 0.03 mm, n=8 cells. (O) Quantification of branch point numbers in class II ldaA cells in control animals: 105.4 ± 7.2, n=11 cells; foxOΔ94 animals: 63.2 ± 3.2, n=11 cells. (P) Quantification of dendrite length in class II ldaA in control animals: 2.76 ± 0.10 mm, n=11 cells; foxOΔ94 animals: 1.81 ± 0.04 mm, n=11 cells. (Q–R) Representative traces of class III vdaD cells of the indicated backgrounds, marked with mCD8-GFP driven by C161-GAL4. (S) Quantification of branch point numbers in class III vdaD cells in control animals: 458.7 ± 29.1, n=10 cells; foxOΔ94 animals: 341.4 ± 10.1, n=10 cells. (T) Quantification of dendrite length in class III vdaD in control animals: 5.12 ± 0.26 mm, n=10 cells; foxOΔ94 animals: 4.57 ± 0.14 mm, n=10 cells. Scale bars in E, I, M, and Q: 50 μm. Error bars are mean ± s.e.m., #, p<0.1, **, p<0.01, ***, p<0.001.