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Comparison of kinetic values of wild-type and mutants of B. anthracis DHOases.
| Enzyme | Substrate | KM (µM) | kcat (s−1) | Kcat/KM (M−1s−1) |
|---|---|---|---|---|
| WT-DHOase | Ca-asp | 112 ± 24 | 2.1 ± 0.1 | 1.9 × 104 |
| WT-DHOase | DHO | 114 ± 16 | 1.9 ± 0.1 | 1.7 × 104 |
| R63A-DHOase | Ca-asp | na | na | na |
| R63A-DHOase | DHO | na | na | na |
| N93A-DHOase | Ca-asp | 1183 ± 251 | 1.4 ± 0.1 | 1.2 × 103 |
| N93A-DHOase | DHO | na | na | na |
| D304A-DHOase | Ca-asp | na | na | na |
| D304A-DHOase | DHO | na | na | na |
na : no activity (not measurable)
