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. Author manuscript; available in PMC: 2017 Feb 28.
Published in final edited form as: Nat Genet. 2016 Aug 29;48(10):1185–1192. doi: 10.1038/ng.3661

Fig. 3. Variant n.-54_-49del found in F454 reduces the activity of the PSE element in dual luciferase assays.

Fig. 3

HeLa cells were transfected with the Promega pGL3 reporter vector carrying the wild type PSE without the deletion (WT U8), with n.-54_-49del (U8 n.-54_-49del), or the reporter vector without an insert (pGL3 empty). The WT U8 PSE vector functioned as a promoter, enhancing luciferase activity by a mean of 109-fold in comparison with empty vector. In contrast, the n.-54_-49del vector demonstrated a mean of 2 fold activity compared to empty vector. Data presented relate to the mean fold change (+/- SD) of relative light units (RLU) compared to the control vector for three independent experiments each with three technical replicates. Data were analyzed using a one way Anova with multiple comparisons where **** = p<0.0001.