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. Author manuscript; available in PMC: 2017 Feb 28.
Published in final edited form as: Nat Genet. 2016 Aug 29;48(10):1185–1192. doi: 10.1038/ng.3661

Fig. 5. 3’ end precursor processing of U8 variants.

Fig. 5

In vitro 3’ end processing of 5’ end labeled in vitro transcribed precursor U8 snoRNA (U8-165) in HeLa nuclear extracts. At 30 minutes, multiple pre-U8 snoRNA processing intermediates can be seen with the wild-type (WT) pre-U8 snoRNA. Four mutants (n.*1C>T, n.*5C>G, n.*9C>T and n.*10C>G) in the pre-U8 snoRNA at 1, 5, 9 and 10 nucleotides downstream of the mature 3’ end of U8 were assessed. All four mutants exhibited a difference in processing intermediates compared to WT. Furthermore, all mutant RNAs displayed additional shorter intermediates which are not present with the WT RNA.. Data are representative of 3 independent experiments.