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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Jul 22;1858(11):2573–2583. doi: 10.1016/j.bbamem.2016.07.008

Figure 2. Log and stationary phase cell cultures performs equivalently in TOXGREEN.

Figure 2

Fluorescence measurements of seven TOXGREEN constructs, including the “no-TM” control. The dashed vertical line indicates the readout wavelength used (512 nm). a) Fluorescence spectra of cells in log phase concentrated 3x and resuspended in PBS buffer. b) Conversion of log phase cell’s fluorescence at 512 nm to TOXGREEN signal. The fluorescence is normalized to cell density and the background fluorescence of the “no TM” construct is subtracted. The signal is here normalized to the GpA sample. c) Spectra of stationary phase cells, measured directly in LB media. d) Conversion of stationary phase cell’s fluorescence to TOXGREEN signal. e) Comparison of fluorescence of log and stationary phase cells after normalization to cell density. f) Comparison of relative TOXGREEN signal for log and stationary phase cells. Wester blots of the relative to these experiment are shown in supplementary Figure S4.