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. 2016 Sep 18;2016:5040528. doi: 10.1155/2016/5040528

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Copyright © 2016 Takako Furusawa et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Identification of apisin by SDS-PAGE and LC-MS. (a) SDS-PAGE profiles of natural RJ (lane 1), the isoelectric point-precipitated protein (pH 4.8) (lane 2), and the protein purified by size-exclusion HPLC (lane 3). (b) SDS-PAGE profile of the protein extracted from the 5 kDa band (lane 1). (c) Total ion chromatogram of the 5 kDa protein. (d) Mass spectra of the 5 kDa protein. (e) Calculated molecular weight of the 5 kDa protein according to MAXENT I analysis. The methodological details are provided in Section 2.