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JMJD2A knockdown decreased the levels of BNP. Cardiac cells were transfected using Lipofectamine RNAiMAX and siRNA. The results of western blot assays using antibodies against JMJD2A and BNP were after 24 hours after transfection. GAPDH was used as the loading control. Data were analyzed for three different experiments which were put together.
